Recombinant full-length human NCK was expressed by baculovirus in Sf9 insect cells using an N-Terminal his epitope. The purity was determined to be >75% by densitometry.
Size
50 μg
Concentration
0.2 μg/μL
Applications
Western Blot. NCK Protein is stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol. NCK Protein is suitable for use in Western Blot. Expect a band approximately ~48kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Other Names
NCK1, MGC12668, NCKalpha, Cytoplasmic protein NCK1 NCK adaptor protein 1, Nck-1, SH2/SH3 adaptor protein NCK-alpha