This product has been prepared from in vitro cell culture by selective precipitation. In an Ouchterlony double diffusion assay the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgM, and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse serum. Typically, less than 1% cross reactivity was detected by ELISA against other mouse isotypes using chain specific antibodies.
Size
100 μg
Concentration
0.5 mg/mL
Applications
FLISA, Flow Cytometry, IF Microscopy. Mouse IgG3 isotype control can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.