Recombinant Human Cell cycle checkpoint protein RAD1 (RAD1)
Description
Purity > 90% as determined by SDS-PAGE. MW: 58.8kDa. Full Length: 1-282aa. N-terminal GST-tagged. Homo sapiens (Human). Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.
Size
100ug
Concentration
n/a
Applications
RUO
Other Names
DNA repair exonuclease rad1 homolog; Rad1-like DNA damage checkpoint protein