Glucose is a ubiquitous energy source in most organisms and plays a pivotal role in cellular metabolisms and homeostasis. Cancer cells exhibit increased glucose uptake to support their high proliferation rate. 2-NBDG (2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl) amino]-D-glucose) is a fluorescent deoxyglucose analog that can be taken up by cells through glucose transporters. However, 2-NBDG cannot be fully utilized in glycolysis because of its modification and thus accumulates inside the cells. Fluorescence generated by this fluorescent glucose analog is proportional to glucose uptake by the cells and can be used to measure glucose uptake using fluorescent microscopy and flow cytometry. To validate the assay, the kit includes phloretin, a
Size
50 assays
Concentration
n/a
Applications
? Measurement of glucose uptake in response to insulin, growth factors, cytokines, mitogens and nutrients, etc.? Dual-staining of glucose transporters and glucose uptake ? Analysis of glucose metabolism and cell signaling in various cell types? Screening of anti-diabetic compounds
Other Names
Glucose uptake assay kit, Cell based Glucose uptake assay, Glucose uptake assay, Cell based Glucose uptake assay kit,