Mouse Specific 2 step biotin-free HRP IHC Detection Kit includes reagents specifically designed for the analysis of antigens in immunohistochemistry and other immunodetection assays. Using polymerization marking method, HRP is conjugated with secondary antibody to form a large molecule antibody-enzyme polymer. The secondary and third antibodies used in traditional methods can thus be replaced by this enzyme polymer. This enzyme polymer is specially fit for immunohistochemistry analysis due to its powerful nature of amplifying signal and permeating tissues and cells. This kit possesses superiority of high speed, high sensitivity, low background and ease-of-use. The provided protocol includes steps for immunohistochemical analysis of antigens with high sensitivity and low background using an unconjugated primary antibody, peroxidase enzyme polymer, and a 5% BSA blocking reagent.
Size
1 kit
Concentration
n/a
Applications
Immunohistochemistry. This kit is ready to use and no dilution is needed. This kit contains 10mls/kit. Number of tests/kit depends on the size of the sample. 100 test/kit if using 100μL/test. This kit is useful for Immunohistochemistry.