This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-MCP-2 antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-MCP-2 antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was cata