Purity ~90% (SDS electrophoresis). Mouse NGF (2.5S) was isolated from mouse submaxillary glands by method of Mobley et al (1976) and is a form of beta-NGF that has identical biological properties. NGF is known to regulate the survival and development of certain sympathetic and sensory neurons. It is a dimer with 2 identical polypeptide chains and dimeric molecular weight of ~26,500Da. Isolation and purification of NGF from mouse submaxillary glands yields preparations of NGF (2.5S) with identical biological activity but with cleavages at the amino terminus (with the loss of 8 amino acids) and/or at the carboxy-terminus (with the loss of arginine). These preparations are named 2.5 NGF Source: Nerve Growth Factor, from mouse Molecular Weight: ~26kD and the monomer ~13kD (SDS-PAGE) Biological Activity: Tested by stimulation of 8 day old chick embryo dorsal root ganglia.