Purity: > 95 % by SDS - PAGE Format: Liquid, In Phosphate buffered saline (pH7.4) containing 20% glycerol, 1mM DTT. MTHFD2 also known as Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial. MTHFD2 protein that possesses three distinct enzymatic activities, methylenetetrahydrofolate dehydrogenase, methenyltetrahydrofolate cyclohydrolase and formate-tetrahydrofolate ligase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. Recombinant human MTHFD2, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.