Intended Use: Gene Regulation: gene expression level, characterization of promoter activity, modulation of gene expression by receptors, transcription factors and small molecules. Drug Discovery: high-throughput screen for gene modulators. Sensitivity: Detection of as little of 2fg luciferase and as few as 4 cells. Sample Type: Mammalian, yeast or E. coli cells Test Principle: The Luciferase Reporter Gene BioAssay? is based on the quantitation of luciferase expression in mammalian, yeast or E. coli cells, using luciferin and ATP as substrates. The reaction results in light production which can be conveniently measured on a luminometer. Reaction scheme: Luciferase ATP+D-luciferin+O2 oxyluciferin+AMP+PPi+CO2+light This bioluminescent reporter gene assay is extremely sensitive and is especially suitable for quantifying luciferase expression in recombinant cells. This ultra-sensitive, homogeneous cell-based assay only requires adding a single reagent to the cells and measuring the li