The competitive 1-Methylhistamine ELISA kit uses the microtiter plate format. 1-Methylhistamine is bound to the solid phase of the microtiter plate. Acylated 1-methylhistamine and solid phase bound 1?methylhistamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free Antigens and free Antigens-antiserum complexes are removed by washing. The antibody bound to the solid phase Antigens is detected by anti-rabbit/peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase Antigens is inversely proportional to the 1-methylhistamine concentration of the sample. Sample Types: urine, plasma, cell culture. Range: 10 - 1000 ng/ml. Sensitivity: 0.13 ng/ml. Time: overnight ? The Eagle Biosciences 1-Methylhistamine ELISA Assay kit is an Enzyme-linked immunosorbent assay used for the quantitative and very sensitive determination of noradrenaline in biological samples including urine, plasma, and cell culture samples. The 1-Methylhistamine ELISA Assay kit is for research use only and should not be used in diagnostic procedures.