Recombinant full length human RAS was expressed in E.coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.
Size
50 μg
Concentration
0.2 μg/μL
Applications
Western Blot. RAS Protein is stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. RAS Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~47kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Other Names
K-ras, N-ras, RASH1, c-bas/has, Harvey rat sarcoma viral oncogene homolog