Recombinant full-length human p53 was expressed by baculovirus in Hi5 cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.
Size
20 μg
Concentration
0.2 μg/μL
Applications
Western Blot. p53 Protein is stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, 10mM glutathione, and 25% glycerol. p53 Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~79kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.