Contains the vitamins as described by Nitsch and Nitsch (1969). Solubility: Water Grams of powder to prepare 1L: 108.6 Instructions for dissolving the powder: Add 5ml 1N KOH or NaOH/100ml package or ~13ml/250ml/package. Stir until suspension dissolves; add additional base if necessary. Bring to volume with water. Storage: This product can be filter sterilized and stored in the refrigerator. Alternatively, it can be stored in aliquots in the freezer for long-term. Freezer storage of non-sterile solutions is preferred as vitamins are subject to breakdown by bacteria and fungi. myo-Inositol may precipitate out of solution when cold; warming with occasional agitation will redissolved the precipitate.