DNA was prepared from cell line WM3629. WM3629 is a metastatic human melanoma cell line that displays a mesenchymal and dendritic morphology. This cell line was established from a lymph node metastasis site of a patient. This cell line contains a D549G mutation in the BRAF gene resulting in an amino acid substitution from aspartic acid to glycine at codon 549 (Asp549Gly). These cells also express G12D mutation in the N-RAS gene resulting in substitution of glycine with aspartic acid at codon 12 (Gly12Asp).WM3629 cells produce xenograft tumors when injected into immunocompromised mice.
Size
10 μg
Concentration
200 ng / μl
Applications
Purified Genomic DNA is suitable for a number of molecular biology applications including but not limited to preparation of genomic libraries, PCR templates, DNA sequencing, DNA fingerprinting, and mutation analysis.
Other Names
Human Melanoma, patient derived tumor, tumor models, skin cancer, xenograft