594 Antibody labeling Kit (50 μg antibody per reaction)
Description
ReadiLink? Rapid iFluor? 594 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*. AAT Bioquest's iFluor? dyes are optimized for labeling proteins, in particular, antibodies. iFluor? 594 dyes have fluorescence excitation and emission maxima of ~590 nm and ~610 nm respectively. iFluor? 594 family has the spectral properties similar to those of Texas Red? and Alexa Fluor? 594 (Texas Red? and Alexa Fluor? 594 are the trademarks of Invitrogen). iFluor? 594 family is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family an excellent alternative to Texas Red? and Alexa Fluor? 594.Compared to Texas Red?, iFluor? 594 is much easier to be conjugated with RPE with much higher conjugation yield, and the resulted RPE-iFluor? 594 tandem has better FRET efficiency.ReadiLink? labeling kits essentially only require 2 simple mixing steps without a column purification needed. iFluor? 594 SE used in this ReadiLink? kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. The kit has all the essential components for labeling ~2x50 ug antibody. Each of the two vials of iFluor? 594 dye provided in the kit is optimized for labeling ~50 μg antibody. iFluor? 594 SE protein labeling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor? 594 SE.