680 Antibody labeling Kit (50 μg antibody per reaction)
Description
ReadiLink? Rapid iFluor? 680 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*. AAT Bioquest's iFluor? dyes are optimized for labeling proteins, in particular, antibodies. These dyes are optimized to have minimal fluorescence quenching effect on proteins and nucleic acids. Our iFluor? 680 dyes have fluorescence excitation and emission maxima close to 680 nm and 700 nm respectively with good photostability. These spectral characteristics make them an excellent alternative to Cy5.5?, IRDye? 700 and Alexa Fluor? 680 (Cy5.5?, IRDye? and Alexa Fluor? are the trademarks of GE Healthcare, Li-COR and Invitrogen respectively). ReadiLink? labeling kits essentially only require 2 simple mixing steps without a column purification needed. iFluor? 680 SE used in this ReadiLink? kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. The kit has all the essential components for labeling ~2x50 ug antibody. Each of the two vials of iFluor? 680 dye provided in the kit is optimized for labeling ~50 μg antibody. iFluor? 680 SE protein labeling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor? 680 SE.