750 Antibody labeling Kit (50 μg antibody per reaction)
Description
ReadiLink? Rapid iFluor? 750 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*. AAT Bioquest's iFluor? dyes are developed for labeling proteins, in particular, antibodies. These dyes are optimized to have minimal fluorescence quenching effect on proteins and nucleic acids. Our iFluor? 750 dyes have fluorescence excitation and emission maxima close to 750 nm and 780 nm respectively with good photostability. Our in-house comparable studies indicated that our iFluor? 750 dyes are significantly brighter than the corresponding Cy7? and Alexa Fluor? 750. These spectral characteristics make them a superior alternative to Cy7? and Alexa Fluor? 750 (Cy7? and Alexa Fluor? are the trademarks of GE Healthcare and Invitrogen respectively). iFluor? 750 conjugates have been widely used in fluorescence animal imaging applications. ReadiLink? labeling kits essentially only require 2 simple mixing steps without a column purification needed. iFluor? 750 SE used in this ReadiLink? kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. The kit has all the essential components for labeling ~2x50 ug antibody. Each of the two vials of iFluor? 750 dye provided in the kit is optimized for labeling ~50 μg antibody. iFluor? 750 SE protein labeling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor? 750 SE.