ReadiLink? Protein Biotinylation Kit *Powered by ReadiView? Biotin Visionization Technology*. Biotin is widely used for labeling biomolecules, in particular, antibodies. This kit is primarily used for the preparation of biotin-labeled IgG for enzyme immunoassay (EIA). Our kit uses our ReadiView? biotin succinimidyl ester (#3059) that reacts with an amino group of IgG and other biomolecules. Our unique biotin contained in the kit carries a color tag for indicating the degree of biotinylation, thus eliminating the troublesome HABA biotinylation determination. This kit contains all of the necessary reagents for labeling and purification. On our hands, 5 to 8 biotin molecules can be conjugated to each IgG molecule using our kit.