Nutrient Mixture Ham's F-12 Modified w/o Cupric Sulfate, Alanine, Aspartic Acid, Glutamic Acid, Glycine, Proline, Hypoxanthine, Inositol, Lipoic Acid, Thymidine. Ham's Nutrient Mixtures were originally developed to support clonal growth of several clones of Chinese hamster ovary (CHO) cells, as well as clones of HeLa and mouse L-cells. Both mixtures were formulated for use with or without serum supplementation, depending on the cell type being cultured. Ham's F-10 has been shown to support the growth of human diploid cells, white blood cells for chromosomal analysis, primary explants of rat, rabbit and chicken tissues. Ham's F-12 has been used for the growth of primary rat hepatocytes and rat prostate epithelial cells. May be used in combination with hormones and transferrin. For optimum buffering, use in a 5% CO2 atmosphere.