Mouse IgG1 Isotype Control (W3/25). The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype control
Size
250 μg
Concentration
n/a
Applications
Flow Cytometry: 10 μL for 10^6 cells in 100 μL, Immunohistochemistry (Frozen): Assay dependent