Human recombinant protein expressed in Nicotiana benthamiana. It is produced by transient expression in non-transgenic plants. This product contains no animal-derived components or impurities. Animal Free product. Interleukin-12 (IL-12) is a heterodimer cytokine composed of p40 and p35 subunits. It is mainly produced by macrophages, dendritic cells, and B cells. It has multiple effects on T and natural killer (NK) cells. In particular it appears to be a major factor for the development of cellular immunity. IL-12 stimulates the production and secretion of several cytokines, in particular IFN-gamma, by NK cells and T cells, induces proliferation and enhances the cytotoxic activity within these cell populations. The p40 subunit of IL-12 interacts with the beta subunit of IL-12R and the disulphide linked p40 homodimer specifically antagonize the activity of bioactive IL-12 in different assay systems. Recombinant human Interleukin-12 p40 is a glycosylated polypeptide chain containing 312 amino acids ( fragment 23 - 328 P29460 IL12B_HUMAN) with an amino- terminal hexahistidine tag. The recombinant protein has a predicted molecular mass of 35.5kDa, however as a result of potential glycosylation, the molecular mass could be approximately 40kDa by SDSPAGE under reducing conditions. Purity >97% by SDS-PAGE gel
-Gee K, et al. (2009) The IL-12 family of cytokines in infection, inflammation and autoimmune disorders. , Mars 8(1):40-52. -Trinchieri, G. (1998) Interleukin 12: a cytokine at the interface of inflame and immunity. Adv. Immunolog., 70: 83-243 -Gillessen, S. et al. (1995) Mouse interleukine-12 (IL-12)p40 homodimer: a potent IL-12 antagonist. Eur. Immunolog, 25:200-206. -Gately, M. K., et al. (1998) The inteleukine-12/interleukine-12 receptor system: role in normal and pathologic immune responses. Annu. Rev. Immunol, 16:495-521 -D'Andrea, A. et al. (1992) Production of natural killer cell stimulatory factor (interleukin 12) by peripheral blood mononuclear cells. J. Exp. Med., 176: 1387-1398. -Chan, S. H. et al. (1991). Induction of interferon gamma production by natural killer cell stimulatory factor: characterization of the responder cells and synergy with other inducers. J. Exp Med., 173:869-879 -Wolf, S. F. et al. (1991) Cloning of cDNA for natural killer cell stimulatory factor, a heterodimeric cytokine with multiple biologic effects on T and natural killer cells. J. Immunol., 146: 3074-3081.