Purity: > 90% by SDS - PAGE Format: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT. CNPY1 is expressed in the midbrain-hindbrain (MHB) boundary in zebrafish, binds FGFR1, and plays a role in FGF signaling. Recombinant human CNPY1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.