Mouse IgA Kappa isotype control was prepared from concentrated cell culture supernatant by a multi-step process which includes selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. In an Ouchterlony double diffusion assay the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgG3 and IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgA alpha and anti-Mouse serum. Light and heavy chain composition confirmed by RID. SDS-PAGE shows a band at 60 kDa corresponding to alpha heavy chain.
Size
1 mg
Concentration
1.0 mg/mL
Applications
ELISA, FLISA, Flow Cytometry, IF Microscopy, Western Blot. Mouse IgA kappa isotype control can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Other Names
Mouse IgA istoype control, Mouse IgA subclass istoype control, Mouse IgA Kappa