The Ansh Labs' Total IGFBP-3 ELISA kit is a quantitative two-step sandwich immunoassay. The Ansh Labs Total IGFBP-3 Assay uses an acidification and neutralization method to dissociate IGFBP-3 from all the binding subunits. Analytical measureable range of 0.10 - 10 ng/mL allows for studies in a variety of physiological states. Sensitive to 0.065 ng/mL. Total 3.5 hour incubation at room temperature. Long shelf life of 24 months. For Research Use Only within the United States. Ansh Labs, located just south of Houston Texas, is an FDA registered manufacturer of diagnostic test kits, antibodies, antigens, and proteins.