The Ansh Labs' Intact IGFBP-3 ELISA is a quantitative two-step sandwich type immunoassay. The immunoassay methods designed for the measurement of bioactive IGF-I, total and Intact IGFBP-3 in patient samples could be of practical value for the diagnosis or prediction of various pathologies including growth abnormalities and cancer. The Intact IGFBP-3 Assay uses an acidification and neutralization method to dissociate Intact IGFBP-3 from all the binding subunits. Intact IGFBP-3 levels are quantified in the extracted samples using a highly sensitive and specific Intact IGFBP-3 ELISA. Total IGFBP-3 levels can be measured using Total IGFBP-3 ELISA (AL-120). Wide dynamic range of 3.5-117 ng/mL. Sensitive to 1.37 ng/mL. Total 1.5 hr incubation at RT. Long shelf life of 24 months. Research Use Only within the United States. Ansh Labs is an FDA registered manufacturer of diagnostic test kits, antibodies, antigens, and proteins.