Purity ≥ 95% (SDS-PAGE) Purified by ion-exchange chromatography and gel-filtration. Applications: Tested in ELISA and immunoblotting with anti-NSE antibodies. Activity: 93umol/min/mg at 25°C Assay Buffer: 45 mM imidazole, pH 6.8, 45 mM potassium chloride, 1.33 mM magnesium sulfate, 1mM 2-PG. Unit Definition: One unit is defined as the amount of NSE converted 1mol of 2-phospho-D-glycerate per minute at 25°C. Sub-unit composition is gamma-gamma.