Purity: > 90% by SDS - PAGE Format: Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing, 1mM DTT, 10% glycerol . Bcl2 modifying factor, also known as BMF belongs to the Bcl2 protein family of apoptosis mediators. Bmf is constitutively expressed in many tissues. This protein contains a single Bcl2 homology domain 3 (BH3), and has been shown to bind Bcl2 proteins and function as an apoptotic activator. Also, BMF is a key molecule for histone deacetylase (HDAC) inhibitors which alters the balance between acetylation and deacetylation, significantly increasing histone acetylation, while strongly inducing apoptosis in a variety of cancer cell types. Recombinant human BMF, fused to T7-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.