Immunogen sequence: L20-C471. Specificity: Natural and recombinant human total MMP13. There is no detectable cross-reactivity with other relevant proteins.
Size
1 kit
Concentration
n/a
Applications
ELISA. Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human MMP13 standard solutions into the pre-coated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma( heparin) to each empty well. It is recommended that each human MMP13 standard solution and each sample be measured in duplicate.