PBMCs are available for various disease states. PBMCs are purified from the blood of donors by Ficoll centrifugation. Fresh blood is collected and treated with a variety of anticoagulants depending on the system used to collect blood. We can typically meet customers' special needs if a specific anticoagulant is preferred--or lack of one. Fresh blood is then carefully applied over the Ficoll in 15 or 50-ml conical tubes and centrifuged without the brake applied. After centrifugation, plasma/serum is removed and we are left with the buffy coat layer, rich in mononuclear cells. The cells are carefully transferred to a new conical tube and washed. Red blood cell lysis can be performed upon request. Cells are then quickly counted, spun down, and resuspended in freezing media (90% FBS + 10% DMSO). Typically PBMCs are frozen in 1mL of freezing media in aliquots of 5-15 million cells/mL. PBMCs are then slowly frozen in isopropyl alcohol containers in -80?C freezers over night. The next day the PBMCs are then put into long term liquid nitrogen storage and banked in our searchable database.