Purity: > 85% by SDS - PAGE Format: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol,1mM DTT . ACAA2 catalyzes the last step of the mitochondrial fatty acid beta-oxidation spiral. unlike most mitochondrial matrix proteins, it contains a non-cleavable amino-terminal targeting signal. Recombinant human ACAA2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.