Amyloid Precursor Protein (APP), Peptide #3, #4 & #5
Clone
polyclonal
Host Species
Chicken
Reactive Species
human, mouse, rat
Isotype
n/a
Format
peptide affinity purified
Size
1000 ul (sampler 200 ul)
Concentration
300 μg/ml
Applications
ICC, IHC
Reviews / Ratings
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Description
n/a
Immunogen
Chickens were immunized with keyhole limpet hemocyanin (KLH) conjugates of three different synthetic peptides (named #3, #4 and #5). Peptide #3 is QHF QEK VES LEQ EAA NER QQ (which corresponds to residues #433-452 of the human sequence NP_000475.1); peptide #4 is EQK DRQ HTL KHF EHV RMV DPK K (residues #501-522); peptide #5 is EEI QDE VDE LLQ KEQ NYS DD (residues #556-575). It should be noted that these three peptide sequences are 100% identical with the mouse (P12023.3) and rat (P08592.1) gene products. After repeated injections, immune eggs were collected from the hens, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified against each of the three cognate peptides using agarose affinity matrices. The eluate concentrations were then adjusted to 300 ug/ml, mixed in equimolar concentrations (100 ug/ml is the final concentration of each antibody), and the preparation was filter-sterilized.