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Description
n/a
Immunogen
Chickens were immunized with a synthetic peptide containing the DYKDDDDK epitope conjugated to keyhole limpet hemocyanin (KLH). The first injection included complete Freund's adjuvant; subsequent injections included incomplete Freund's adjuvant. Eggs were collected after the fourth injection, and antibodies were purified from the yolks using a proprietary method that yields a purity of >90%. Antibodies were then affinity-purified over a peptide column and dialyzed against phosphate-buffered isotonic saline. Sodium azide was added, the protein concentration was adjusted to 100 mg/ml, and the antibodies were filter-sterilized (0.2 μm).