human, monkey, mouse, rabbit, rat, bovine, porcine
Isotype
n/a
Format
affinity purified
Size
100μg
Concentration
0.5 mg/mL
Applications
ELISA, RIA
Reviews / Ratings
If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY.
Description
This affinity?purified polyclonal antiserum was specifically prepared to provide broad cross?reactivity with human insulin and common insulin analogs to allow use in ELISAs/RIAs and as an immunoaffinity capture reagent in LC?MS/MS assays. The immunogen consisted of pharma?grade insulin. Following immunization and boosting, the guinea pig antiserum was purified by a multi?stepped process which culminated in an affinity column procedure to yield the broad?specificity antiserum. This antiserum will work exceptionally well as a capture or detection reagent in a conventional noncompetitive ELISA format. Because of its polyclonal nature, the antiserum can be used as both a capture and detection reagent. We recommend either biotinylation or another conjugation procedure be used for the detection reagent. Suggested starting dilutions are: ELISA: 1/10,000 - 1/50,000, RIA: 1/200,000, and Immunoaffinity capture: 1/10,000 - 1/50,000