Purity ~80% (SDS-PAGE). Lck-interacting transmembrane adapter 1 precursor, also known as LIME1, was previously characterized as a transmembrane adaptor protein mediating TCR-dependent T cell activation. LIME1 becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME1 associates with the Src family kinase Lck and its negative regulator, Csk. Source: Recombinant protein corresponding to aa28-295 from human LIME1, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~30.8kD (291aa) Applications: Suitable for use in SDS-PAGE.