HN-mb are isolated from human midbrain (mesencephalon). HN-mb are cryopreserved as primary cultures and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. HN-mb are characterized by immunofluorescence with antibodies specific to neurofilament, MAP2, and ?-tubulin III. HN-mb are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HN-mb are guaranteed to further culture under the conditions provided by Creative Bioarray; however, HN-mb are not recommended for expanding or long-term cultures since the cells do not proliferate in culture.