1,5-Anhydroglucitol Uptake Assay Kit utilizes a proprietary fluorescent 1,5-AG analog, which can be taken up by cells. However, this 1,5-AG analog cannot be fully utilized in glycolytic processes and thus accumulates inside the cells. Fluorescence generated by this analog is proportional to the cellular 1,5-AG uptake. The assay can be used to monitor 1,5-AG uptake by using fluorescent microscopy and flow cytometry. Phloretin, a compound that can inhibit glucose transporter and 1,5-AG cell uptake, is included in the kit as a control. This non-radioactive assay kit is easy-to-use and allows qualitative and quantitative measurements of 1,5-AG uptake in cultured cells.
Size
50 assays
Concentration
n/a
Applications
Measurement of 1,5-Anhydroglucitol uptake in response to insulin, growth factors, cytokines, mitogens and nutrients, etc.Dual-staining of 1,5-AG transporters and 1,5-AG uptake Analysis of 1,5-AG metabolism and cell signaling in various cell typesScreening of anti-diabetic compounds