Flow Cytometry, Immunohistochemistry (IHC), Frozen Sections, Immunofluorescence, Immunoprecipitation / Cell separatiopn, Western Blot
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Description
Fibroblast marker: The monoclonal antibody clone reacts specifically with human CD90 (Thy-1). No reaction with rat, mouse, rabbit, monkey. CD90 (Thy-1) in human is primarily expressed by nerve cells, additionally in a sub-population (20%) of CD34+ blood stem cells an in various fibroblasts. AS02 recognizes fibroblasts of different origin, but does not react with human blood cells, keratinocytes, resting micro-/macrovascular endothelial cells and components of the extracellular matrix like fibronectin, collagen type I, III, IV and laminin. Thus, AS02 is especially suitable for the specific detection and cell separation of human fibroblasts.
A ligand of human thy-1 is localized on polymorphonuclear leukocytes and monocytes and mediates the binding to activated thy-1-positive microvascular endothelial cells and fibroblasts. Saalbach A, Haustein UF, Anderegg U. J Invest Dermatol 2000 Nov;115(5):882-8. Detection of human soluble Thy-1 in serum by ELISA. Fibroblasts and activated endothelial cells are a possible source of soluble Thy-1 in serum. Saalbach A, Wetzig T, Haustein UF, Anderegg U. Cell Tissue Res 1999 Nov;298(2):307-1. The monoclonal antibody AS02 recognizes a protein on human fibroblasts being highly homologous to Thy-1. Saalbach A, Kraft R, Herrmann K, Haustein UF, Anderegg U. Arch Dermatol Res 1998 Jul;290(7):360-6. The fibroblast-specific MAb AS02: a novel tool for detection and elimination of human fibroblasts. Saalbach A, Aust G, Haustein UF, Herrmann K, Anderegg U. Cell Tissue Res 1997 Dec;290(3):593-9. Novel fibroblast-specific monoclonal antibodies: properties and specificities. Saalbach A, Anderegg U, Bruns M, Schnabel E, Herrmann K, Haustein UF. J Invest Dermatol 1996 Jun;106(6):1314-9.