IHC, Paraffin Sections (FFPE), Frozen Sections, Western Blot, Immunocytochemistry
Reviews / Ratings
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Description
During cardiogenesis two major isoforms of myosin light chain 2 are co-expressed in a tightly regulated manner. MLC-2V is only present in the ventricle while MLC-2A is exclusively expressed in the atrium. Knock out studies revealed that the 2A isoform cannot substitute for the 2V variant in the ventricular chamber. Recently it has been demonstrated that embryonic and adult stem cells can be differentiated into cardiomyocytes which may generate suitable replacements for damaged heart tissue in the future. These monoclonal antibodies (#SySy310111 & #SySY311011) are useful tools to distinguish between ventricle and atrium specific cardiomyocytes.
Immunogen
Recombinant protein of human myosin light chain 2A.
Human platelet lysate as a fetal bovine serum substitute improves human adipose-derived stromal cell culture for future cardiac repair applications. Naaijkens BA, Niessen HW, Prins HJ, Krijnen PA, Kokhuis TJ, de Jong N, van Hinsbergh VW, Kamp O, Helder MN, Musters RJ, van Dijk A & Juffermans LJ. Cell and Tissue Research 348: 119-30, 2012. / Functional cardiomyocytes derived from human induced pluripotent stem cells. Zhang J, Wilson GF, Soerens AG, Koonce CH, Yu J, Palecek SP, Thomson JA & Kamp TJ. Circulation Research 104: e30-41, 2009. / Cardiomyocyte enrichment from human embryonic stem cell cultures by selection of ALCAM surface expression. Rust W, Balakrishnan T & Zweigerdt R. Regenerative Medicine 4: 225-37, 2009. / Human cardiomyocyte progenitor cell transplantation preserves long-term function of the infarcted mouse myocardium. Smits AM, van Laake LW, den Ouden K, Schreurs C, Szuhai K, van Echteld CJ, Mummery CL, Doevendans PA & Goumans MJ. Cardiovascular Research 83: 527-35, 2009.