The determination of albumin is carried out as direct competitive ELISA. Bovine albumin has been pre-coated onto a microplate. During incubation the binding of an enzyme-linked anti-albumin (bovine)-antibody to the wells is inhibited by albumin from samples/standards. After washing away any unbound antibody, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is reverse proportional to the albumin con cen tration. Sample Types: serum, urine, biological samples. Range: 10 - 40,960 ng/ml. Sensitivity: 50 ng/ml. Time: 2.5 hours ? This Bovine Albumin ELISA Assay Kit furthermore allows quantification of albumin in bovine urine to estimate kidney function. When blood is filtered in glomeruli of kidneys, highly molecular substances are held back. There fore, urine is nearly protein free. In case of kidney damages, small proteins like albumin appear in urine. Research has demonstrated that albumin therefore serves as a marker in moni toring kidney damages.