Eagle Biosciences Androstenedione Saliva ELISA Assay Kit is a competitive immunoenzymatic colorimetric method for quantitative determination of Androstenedione concentration in saliva. Androstenedione Saliva ELISA Assay Kit is intended for research use only. Sample Types: saliva. Range: 20 - 1000 pg/ml. Sensitivity: 0.5 pg/ml. Time: 1.5 hours ? Androstenedione has relatively weak androgenic activity, estimated at ~ 20% of testosterone. Secretion and production rates also exceed those of testosterone in women in whom significant extra-adrenal conversion of androstenedione to testosterone occurs. In premenopausal women the adrenal glands and ovaries each produce about half of the total androstendione (about 3 mg/day). After menopause androstenedione production is about halved, primarily due to the reduction of steroid secreted by the ovary. Nevertheless, androstenedione is the principal steroid produced by the postmenopausal ovary. The high serum-saliva correlation for androstenedione suggests that individual differences in serum androstenedione levels may be accurately estimated using saliva as a non-invasive alternative specimen.