The ImmunoGuide Antibody to Cetuximab ELISA is a sandwich type ELISA for the determination of free antibodies against Cetuximab in serum and plasma samples. During the first incubation period, antibodies to Cetuximab in patient serum/plasma samples are captured by the drug Cetuximab coated on the wall of the microtiter wells. After washing away the unbound components from samples, a peroxidase-labelled Cetuximab conjugate is added and then incubated. Antibody to Cetuximab, if present in sample, will make a bridge, with its two identical Fab arms, between the Cetuximab coated on the well and the other Cetuximab labeled with peroxidase. After a second washing step, the bound enzymatic activity is detected by addition of tetramethylbenzidine (TMB) chromogen-substrate. Finally, the reaction is terminated with stop solution. The positive reaction is related with the presence of antibodies to Cetuximab in the sample. Sample Types: serum, plasma. Range: Cut-off. Sensitivity: Cut-off. Time: 2.5 hours ? The Eagle Biosciences Antibody to Cetuximab is an enzyme immunoassay for the semi-quantitative determination of free antibodies to Cetuximab in serum and plasma samples. The Antibody to Cetuximab ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
Size
1 x 96 well
Concentration
n/a
Applications
RUO
Other Names
anti-drug antibody kits, anti drug shikari shakari , Erbitux Ab, Antibodies, Antibody, ELISA Assay Kit EIA, Immunoassay, Cetuximab, IG-BB112, Tani