An enzyme immunoassay for the quantitative measurement of total human chorionic gonadotropin (hCG and b-hCG) in serum. This product is for Research Use Only; International customers contact your representative to receive information about the CE marked version. The DRG Beta-HCG ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal antibody [mouse] directed towards a unique antigenic site on a Beta-HCG molecule. An aliquot of sample containing endogenous Beta-HCG and/or HCG is incubated in the coated well with enzyme conjugate, which is an anti- Beta-HCG antibody conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of Beta-HCG/HCG in the sample. Having added the substrate solution, the intensity of color developed is proportional to the concentration of Beta-HCG/HCG in the sample.