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Description
This antibody was cross-adsorbed to phospho-β-Catenin (Tyr-654) peptide before affinity purification using phospho-β-Catenin (Tyr-489) peptide (without carrier). The antibody detects 84 and 88kDa proteins corresponding to the molecular mass of γ-Catenin and β-Catenin, respectively, on SDS-PAGE immunoblots of A431 cells treated with pervanadate, but does not detect these proteins in control cells.
Immunogen
Phospho-β-Catenin (Tyr-489) synthetic peptide corresponds to amino acid residues around tyrosine 489 of human β-Catenin. This peptide sequence is highly conserved in rat and mouse β-Catenin, and has high homology to the conserved site in γ-Catenin (Tyr-480).