Exosomes and other extracellular vesicles (EVs) are small membrane vesicles containing protein, mRNA, micro RNA, DNA and lipids, which are secreted by various cells and are stable in body fluids including blood, saliva, urine, cerebrospinal fluid (CSF) and breast milk. These EVs have been recognized as messengers of cell-to-cell communication, and biomarkers for various diseases. Conventionally, ultracentrifugation, affinity purification using antibody to surface antigen, and precipitation with polymer reagents are used for isolation of exosomes. However, these methods are not fully satisficing in all performances criteria such as recovery efficiency, purity and operability. MagCapture Exosome Isolation Kit PS adopts a novel affinity purification method using magnetic beads and phosphatidylserine (PS)-binding protein (PS affinity method). This kit can easily isolate high purity exosomes and other EVs from cell culture medium and body fluids at high yield by a normal microcentrifuge. If higher purity exosomes are needed, please use the 10,000 x g supernatant as sample. This kit enables the isolation of exosomes and other EVs as intact forms because the captured EVs are eluted from magnetic beads with metal-chelating reagent at neutral pH. The isolated intact exosomes and other EVs can be used for various applications including electron microscopic analysis, nanoparticle tracking analysis, administration of EVs and analysis of molecular constituents such as proteins, lipids, or nucleic acids.