The IDS Rat/Mouse PINP EIA kit is a competitive enzymeimmunoassay utilising a polyclonal rabbit anti-PINP antibody coated onto the inner surface of polystyrene microtitre wells. Calibrators, controls and samples are added to the wells of the microtitre plate followed by PINP labelled with biotin and the plate incubated for 1 hour at room temperature before aspiration and washing. Enzyme (horseradish peroxidase) labelled avidin is added and binds selectively to complexed biotin and, following a further wash step, colour is developed using a chromogenic substrate (TMB). The absorbance of the stopped reaction mixtures are read in a microplate plate reader, colour intensity developed being inversely proportional to the concentration of PINP.
Size
96 wells
Concentration
n/a
Applications
colorimetric ELISA or EIA
Other Names
N-terminal propeptide of type I procollagen, PINP, P1NP kit, assay, immunoassay