Serum Pre-Clinical CartiLaps? (CTX-II) ELISA is based on binding of two identical monoclonal antibodies to cross-linked serum fragments of type II collagen. Initially, biotinylated monoclonal antibody are bound to the surface of streptavidin-coated wells of the microtitre plate. After washing, standards, controls, serum samples and Incubation buffer are pipetted into the wells. After incubation the wells are washed, and a solution of peroxidase-conjugated monoclonal antibody is added to the wells. Following the third washing step, a chromogenic substrate is added to all wells and the colour reaction is stopped with sulphuric acid and the absorbance is measured.
Size
96 wells
Concentration
n/a
Applications
colorimetric ELISA or EIA
Other Names
degradation products of C- terminal telopeptides of type II collagen (CTX-II) kit, assay, immunoassay