The Urine BETA CrossLaps? (CTX-I)ELISA is based on two highly specific monoclonal antibodies against the amino acid sequence of EKAHD-β-GGR, where the aspartic acid residue (D) is β-isomerized. In order to obtain a specific signal in the Urine BETA CrossLaps? (CTX-I)ELISA, two chains of EKAHD-β-GGR must be cross-linked. Standards, controls, or unknown urine samples are pipetted into the appropriate microtitre wells coated with streptavidin, followed by application of a mixture of a biotinylated antibody and a peroxidase-conjugated antibody. Then, a complex between CrossLaps antigens, biotinylated antibody and peroxidase-conjugated antibody is generated, and this complex binds to the streptavidin surface via the biotinylated antibody. Following the one-step incubation at room temperature, the wells are emptied and washed. A chromogenic substrate is added and the colour reaction is stopped with sulfuric acid. Finally, the absorbance is measured.
Size
96 wells
Concentration
n/a
Applications
colorimetric ELISA or EIA
Other Names
crosslaps, degradation products of C-terminal telopeptides of Type-I collagen kit, assay, immunoassay