The Urine CrossLaps? (CTX-I)EIA assay is based on the competitive binding of the anti-CrossLaps antibodies to either soluble CrossLaps antigen or to CrossLaps antigen-coated micro-titre wells. Standards, controls, or unknown samples are pipetted into the appropriate microtiter wells. Then the anti-CrossLaps antibodies are added and incubation takes place for 1 hour at 18-22°C. The wells are washed and peroxidase conjugated anti-rabbit immunoglobulin is added. The wells are then incubated again for 1 hour at 18-22°C. After a second washing step the wells are incubated for 15 min with a chromogenic substrate. The reaction is stopped and the absorbance is measured.
Size
96 wells
Concentration
n/a
Applications
colorimetric ELISA or EIA
Other Names
crosslaps, C-terminal telopeptides of Type I collagen; CTX-I kit, assay, immunoassay