The Urine Pre-Clinical CartiLaps? (CTX-II) EIA is a modification of the Urine CartiLaps? EIA (Christgau (2001), which is based on the competitive binding of a monoclonal antibody to urinary fragments of type II collagen or to biotinylated, synthetic peptides bound to the surface of microtitre plates coated with streptavidin. The modification allows a broadening of the measuring range facilitating the measurement of non-human urines having a wide range of CTX-II values. Initially, biotinylated, synthetic peptides are bound to the surface of streptavidin-coated wells of the microtitre plate. After washing, standards, controls and urine samples are pipetted into the wells followed by addition of a solution of the monoclonal antibody. The wells are washed, and a solution of peroxidase-conjugated anti-mouse immunoglobulin (rabbit) is added to the wells. Following the second washing step, a chromogenic substrate is added to all wells and the colour reaction is stopped with sulphuric acid and the absorbance is measured.
Size
96 wells
Concentration
n/a
Applications
colorimetric ELISA or EIA
Other Names
degradation products of C-terminal telopeptides of type II collagen kit, assay, immunoassay