dove, eel, goldfish, hamster, human, monkey, mouse, sheep, rat, trout
Isotype
n/a
Format
serum
Size
100 μL
Concentration
n/a
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot
Reviews / Ratings
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Description
The ACTH Antibody was raised to ACTH (1-39) purified from porcine pituitary. The antibody produces a maximum fluorescein staining at a 1/100 - 1/200 dilution and a 4+ biotin-streptavidin/HRP staining at a 1/500 - 1/1000 dilution in rat anterior/intermediate pituitary. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. The recommened dilution series are 1/100-1/200 in PBS/0.3% Triton X-100 - FITC Technique and 1/500-1/1000 in PBS/0.3% Triton X-100 - Bn-SA/HRP Technique. Staining is completely eliminated by pretreatment of the diluted antibody with 100 μg/mL of ACTH.